Lentiviral vectors (LVs) are gene delivery vehicles for the genetic modification of mammalian cells. They integrate into the host cell genome, providing a stable, long term expression of the gene of interest (GoI) sequence with very low genotoxic potential. In contrast to gamma-retroviral vector systems, LVs transduce both non-dividing as well as actively dividing cell types, leading to highly efficient transduction of cell populations of interest.
Lentiviral vectors designed for clinical use are derived from HIV-1. The vectors do not express any HIV genes and are replication incompetent. After binding to the cell surface and internalization, the RNA-genome is converted to DNA which then stably integrates into the host cell chromosomal DNA. Finally, the genetic sequence of interest- e.g. a shRNA, miRNA or gene-is transcribed by the cell‘s intrinsic transcription machinery.
Lentigen utilizes proprietary third generation SIN vector system that has been designed to enhance clinical translation capabilities. The vectors can be pseudotyped with a range of envelope proteins, depending upon the specific application.
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